Vertebrate Robo3 / Rig1 is also alternatively spliced; its two splice products are expressed at different times during commisural axon guidance, and have opposing activities.
2.
There are also inputs from the medial septum and from the diagonal band of Broca which terminate in the stratum radiatum, along with commisural connections from the other side of the hippocampus.
3.
Robo binds directly to the cytoplasmic domain of DCC and experiments with Xenopus explants have shown that this interaction silences netrin-mediated attraction; however, in vivo experiments have not yet confirmed the relevance of this mechanism for commisural axon guidance in embryos.
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