1. Payen discovered the first enzyme, diastase , in 1833. 2. French chemist Anselme Payen was the first to discover an enzyme, diastase , in 1833. 3. The presence of the glycogen can be demonstrated with positive PAS staining and negative PAS diastase staining. 4. The diastase negative slide will show a magenta staining where glycogen is present within a section of tissue. 5. The slide that has been treated with diastase will lack any positive PAS staining in those locations on the slide 6. Bee digestive enzymes-invertase, amylase, and diastase -and gastric acid hydrolyze sucrose to a mixture of glucose and fructose. 7. The enzyme was originally intended for use in distilleries, but was more successfully marketed as " Taka-diastase " for dyspepsia. 8. PAS diastase stain ( PAS-D ) is PAS stain used in combination with diastase, an enzyme that breaks down glycogen. 9. PAS diastase stain ( PAS-D ) is PAS stain used in combination with diastase , an enzyme that breaks down glycogen. 10. Overheating is indicated by change in enzyme levels, for instance, diastase activity, which can be determined with the Schade or the Phadebas methods.
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