Electroendosmosis is a reason agarose is used preferentially over agar as agaropeptin in agar contains a significant amount of negatively charged sulphate and carboxyl groups.
2.
Electroendosmosis is a reason agarose is used in preference to agar as the agaropectin component in agar contains a significant amount of negatively charged sulphate and carboxyl groups.
3.
These negatively charged groups create a flow of water in the opposite direction to the movement of DNA in a process called electroendosmosis ( EEO ), and can therefore retard the movement of DNA and cause blurring of bands.
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