Constructing human colorectal cancer cell line with stably down - regulated grp94 ( 1 ) the plasmid prc / rsv - ribol that contains specific grp94 - targeting ribozyme and the control plasmid prc / rsv were miniprepared , respectively , cleaved by endoenzyme pvuii
2.
This time , using cdna of zmcdc5 as template , we amplify a sequence by means of pcr technology . and then , using restrict endoenzyme and ligase , we conjunct the 0 . 8kb length dna sequence in a expression vector , pet - 30a . after induction , expression and purification , we obtained a 35 . 4kda truncated fusing zmcdc5 protein which contains 267aa ( 647 to 914aa in zmcdc5 ) . with the purified protein , we got its antibody and testified the antibody by means of western blotting and dot blotting
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