This action stabilizes the enediol intermediate and the other transition states on the reaction pathway.
2.
The reaction proceeds in such a way that trans-2, 3-enediol phosphate is the intermediate.
3.
The ring opening step may be catalyzed by His99 and Asp270, and isomerization is likely a cis-enediol mechanism.
4.
This isomerization of a ketose to an aldose proceeds through an " cis "-enediol ( ate ) intermediate.
5.
Then, the intermediate is decarboxylated, yielding a 1, 2-enediol of ribulose 5-phosphate, which tautomerizes to form ribulose 5-phosphate.
6.
Besides the precisely placed glutamate and histidine residues to form the enediol, a ten-or eleven-amino acid chain of TPI acts as a loop to stabilize the intermediate.
7.
Rough mechanism showing cis-enediol intermediateMPI must convert an aldose ( mannose ) to a ketose ( fructose ), in addition to opening and closing the rings for these sugars.
8.
In carbohydrate chemistry, the Lobry de Bruyn van Ekenstein transformation is the base or acid-catalyzed transformation of an aldose into the ketose isomer or vice versa, with a tautomeric enediol as reaction intermediate.
9.
In the reaction, the overall consequence is the movement of a carbonyl group from carbon number 1 to carbon number 2; this is achieved by the reaction going through an enediol intermediate ( Figure 1 ).
10.
It was expected that solvent protons would contribute to forming the product from the enediol intermediate of the proton-transfer mechanism and when such contributions were not observed in fluorinated on the methyl group and deuterated on the aldehyde.
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