1. The luciferin -luciferase reaction responsible for the bioluminescence is pH sensitive. 2. Like all luciferins , it is oxidised to produce light . ]] 3. Firefly luciferase generates light from luciferin in a multistep process. 4. Luciferin -luciferase reactions are not the only way that organisms produce light.5. First, D-luciferin is tautomerizes between the keto-enol form. 6. This shows that luciferase can differentiate between the isomers of the luciferin structure. 7. Calcium triggers release of the luciferin ( coelenterazine ) from the luciferin binding protein. 8. Calcium triggers release of the luciferin ( coelenterazine ) from the luciferin binding protein. 9. Luciferin reacts with oxygen to create light:10. Hirata tasked him with purifying crystallizing Cypridina luciferin for the purpose of determining its structure.