1. Nitrification could potentially become a " bottleneck " in the nitrogen cycle.2. Fill CBOD bottle with CBOD dilution water and Nitrification Inhibitor. 3. Therefore, complete nitrification is usually achieved in the B-stage. 4. The conventional removal is nitrification , followed by denitrification. 5. To prevent fish deaths, nitrification via aeration prior to discharge is often desirable. 6. "' Nitrification "'is the biological autotrophic bacteria and archaea. 7. Nitrification is important in agricultural systems, where fertilizer is often applied as ammonia.8. Nitrification also plays an important role in the removal of nitrogen from municipal wastewater.9. Some inhibitors of nitrification also inhibit the production of methane, a greenhouse gas. 10. It is a two-step process, consisting of nitrification followed by denitrification.