1. Hybrids may also be produced by a technique called protoplast fusion. 2. To create knockout moss, transfection of protoplasts is the preferred method. 3. These are tubelike structures that connect the protoplast with the cell wall. 4. In this case protoplasts are fused, usually in an electric field. 5. Gram-positive bacteria are called protoplasts when they lose their cell walls. 6. Protoplasts are widely used for DNA regeneration.7. Protoplasts may also be used for plant breeding, using a technique called protoplast fusion.8. Protoplasts may also be used for plant breeding, using a technique called protoplast fusion. 9. The DNA construct is then incubated with moss protoplasts in the presence of polyethylene glycol. 10. The locally present Veneti and Rugians became influenced by the Goths or their Scandinavian protoplasts .
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