1. The priming event on the lagging strand establishes a replication fork. 2. Human PrimPol ( ccdc111 and in restarting stalled replication forks. 3. Topoisomerases are responsible for removing these supercoils ahead of the replication fork. 4. Primosomes are nucleoproteins assemblies that activate DNA replication forks. 5. DNA damage prevents the normal enzymatic synthesis of DNA by the replication fork. 6. All other proteins at the replication fork are linked directly or indirectly to DnaB. 7. The DNA helicases and polymerases must remain in close contact at the replication fork. 8. Termination requires that the progress of the DNA replication fork must stop or be blocked. 9. Gyrase is most commonly found upstream of the replication fork, where the supercoils form. 10. Spatial juxtaposition of replication sites brings "'clustering "'of replication forks.