11. The albumin remains in the supernatant fraction during the solid / liquid extraction under these conditions. 12. Bottles with hemolytic supernatant were discarded. 13. The supernatant , known as clear juice is drawn off of the clarifier and sent to the evaporators. 14. There are many standardized cell lines available and supernatant is much simpler to analyze than proximal body fluid. 15. Next, phenanthridine is added to the supernatant and this is also mixed at 55 �C for 16 hours. 16. Purification of protein from bovine-derived stromal cell supernatants produces a substantially homogeneous factor, free of extraneous materials. 17. This maintains the well-being of the hybridomas and provides enough cells for cryopreservation and supernatant for subsequent investigations. 18. The supernatant water is then run back into the treatment process or disposed of as a waste-water stream. 19. This removes some of the salts present in the leftover supernatant and bound to DNA pellet making the final DNA cleaner. 20. The plasmid-containing supernatant is carefully removed and can be further purified or used for analysis, such as gel electrophoresis.
