1. Cancer cell line supernatant is an attractive source of secreted proteins. 2. The supernatant fluid is tested for agglutinating activity for guinea pig erythrocytes. 3. Following centrifugation, the supernatant is discarded and the sediment is vortexed. 4. If the diluent is unknown, equilibrium supernatant is readily obtained by centrifugation. 5. On top of the sand bed sits a supernatant layer of unpurified water. 6. The toxin concentration of the supernatant is proportional to the organism cell count. 7. There is only one justified way to perform this dilution-by using equilibrium supernatant . 8. The supernatant containing contaminants can be carefully removed so as not to disturb the beads. 9. The supernatant liquid ( containing many of the fission products ) was separated from the solid. 10. This suspension is centrifuged again to once again pellet DNA and the supernatant solution is removed.
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