21. RACGAP1 also interacts with PRC1 to stabilize and maintain the central spindle as anaphase proceeds. 22. PRC1 is normally inhibited until anaphase onset by CDK1 mediated phosphorylation, preventing its dimerization. 23. Required for chromosome segregation at metaphase-anaphase transition, robust mitotic spindle formation and cytokinesis. 24. "Anaphase lag " occurs when the movement of one chromatid is impeded during anaphase. 25. "Anaphase lag " occurs when the movement of one chromatid is impeded during anaphase . 26. Anaphase starts when the anaphase promoting complex marks an inhibitory chaperone called securin with ubiquitin for destruction.27. Anaphase starts when the anaphase promoting complex marks an inhibitory chaperone called securin with ubiquitin for destruction. 28. This would be accomplished by regulation of the anaphase -promoting complex, securin, and separase. 29. Figure 4 : Potential network diagram involving securin and separase to generate switch-like activation of anaphase 30. Overexpression of cyclin A2 in mammalian cells can result in the delayed onset of metaphase and anaphase .
