For fluorescence-detection size-exclusion chromatography-based thermostability assay ( FSEC-TS ) the samples are heated in the same manner as in FastPP and CETSA and after centrifugation to clear away precipitate the supernatant is treated in the same manner as FSEC . Larger aggregates are seen in the void volume while the peak height for the protein of interest decreases when the unfolding temperature is reached.
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