A more stringent test would involve following the chemical shifts of each and every atom in the molecule by nuclear magnetic resonance ( NMR ) as a function of temperature / denaturant.
32.
It is not generally known how the denaturant affects the protein's refolding, and it is difficult to experimentally determine if these denatured states contain residual structures which may influence folding behavior.
33.
Fluorescence spectroscopy can be used to characterize the equilibrium unfolding of proteins by measuring the variation in the intensity of fluorescence emission or in the wavelength of maximal emission as functions of a denaturant value.
34.
A "'chevron plot "'is a way of representing protein folding chevron shape observed when the logarithm of the observed relaxation rate is plotted as a function of the denaturant concentration.
35.
In practice, the observed experimental data at different denaturant concentrations are fit to a two-state model with this functional form for \ Delta G, together with linear baselines for the folded and unfolded states.
36.
Natural gasoline is often used as a denaturant for Fuel Grade Ethanol where it is commonly added volumetrically between 2.0 % and 2.5 % to make Denatured Fuel Ethanol ( DFE ) or E98.
37.
In 1960 US patents were filed and shortly thereafter Bitrex was approved as an ethanol denaturant by the US Treasury Department's Bureau of Alcohol Tobacco & Firearms ( now Alcohol and Tobacco Tax and Trade Bureau ).
38.
The conformational stability \ Delta G can be calculated for any denaturant concentration ( including the stability at zero denaturant ) from the fitted parameters m and \ left [ D \ right ] _ { 1 / 2 }.
39.
The conformational stability \ Delta G can be calculated for any denaturant concentration ( including the stability at zero denaturant ) from the fitted parameters m and \ left [ D \ right ] _ { 1 / 2 }.
40.
The "'denaturant binding model "'assumes that there are specific but independent sites on the protein molecule ( folded or unfolded ) to which the denaturant binds with an effective ( average ) binding constant " k ".
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