41. Succinate is an intermediate of the TCA cycle, which metabolizes glucose . crotonyl-coenzyme A ( CoA ).42. This gene encodes a major catalytic subunit of succinate -ubiquinone oxidoreductase, a complex of the mitochondrial respiratory chain. 43. The complex is a 4 subunit membrane-bound lipoprotein which couples the oxidation of succinate to the reduction of ubiquinone. 44. Further, shift from glucose to succinate as the carbon source resulted in a long lag period and slow growth rate. 45. The systematic name of this enzyme class is "'succinate -semialdehyde : NAD + oxidoreductase " '. 46. Penicillin N + 2-oxoglutarate + O 2 \ rightleftharpoons deacetoxycephalosporin C + succinate + CO 2 + H 2 O 47. The activity of succinate dehydrogenase is readily observed by the naked eye as the solution turns from colourless to rusty red. 48. :4-methylaminobutanoate + O 2 + H 2 O \ rightleftharpoons succinate semialdehyde + methylamine + H 2 O 2 49. However, when overexpressed, it can replace either DcuA or DcuB in catalysing fumarate-succinate exchange and fumarate uptake. 50. Thus the succinate just adds to the population of circulating molecules in the cycle and undergoes no net metabolization while in it.
