These thickness are sometimes achievable with the use of an ultramicrotome.
2.
An ultramicrotome is a main tool of ultramicrotomy.
3.
A serial block-face scanning electron microscope consists of an ultramicrotome mounted inside the vacuum chamber of a scanning electron microscope.
4.
Following imaging the ultramicrotome is used to cut a thin section ( typically less than 30 nm ) from the face of the block.
5.
Many different samples can be prepared for SBFSEM and the ultramicrotome is able to cut many materials, therefore this technique has wider applicability.
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Finally, thin sections are cut with a glass or diamond knife using an ultramicrotome and the sections are left floating on water that is held in a boat or trough.
7.
After flying over Angel Falls in his home country of Venezuela he was inspired by the concept of the smoothly reoccurring flow system inherent in a waterfall to take his diamond knife invention and combine it with an ultramicrotome to dramatically improve the ultra-thin sectioning of electron microscopy samples.
8.
Smith has also been a primary leader in the development of array tomography ( AT ), a proteomic imaging method of volumetric microscopy, in which ultrathin sections of a plastic-embedded tissue are sliced using an ultramicrotome, bonded in an ordered array to a glass coverslip, stained, and imaged.
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